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Variant 3: Directed mapping

  
Figure 4: The expected number of contigs after n steps using a directed strategy. It's Fig 6. in Nelson and Speed (1994).

Instead of using the random approaches such as bottom-up fingerprinting and STS-content mapping, Mizukami et al and Palazzolo et al ([12], [14]) proposed a directed approach to construct the maps. Roughly speaking, directed approaches proceed sequentially by repeatedly

The process continues until all clones have been either selected as seed clones or identified as overlapping some selected clone. Under this scheme, suppose we have a random library of N clones of length with mean L for a genome of length G. The expected number and size of contigs and the expected proportion of the genome left uncovered after step n can be computed ``exactly'' for the case of constant clone length, and approximately by the similar thinning arguments. For the formulas and details, see [10]. Interestingly, the progression of the project (the number of contigs) behaves quite differently from the random strategies. See Figure 4(Fig 6. in Nelson and Speed [10]). Unlike the long right-hand tail in the bottom-up random schemes, a project using a directed strategy has a slower progress in the beginning, but closes the gaps much faster in later stages.



Simon Cawley
Thu Apr 30 03:30:28 PDT 1998