Stat 260: Statistics in Genetics

Ru-Fang Yeh

Week 10: Clones and clone maps

Stat 260: Statistics in Genetics

Ru-Fang Yeh

Week 10: Clones and clone maps

• Introduction
• Theory
  • Basic coverage result: random clones of constant length, fixed point
  • More sophisticated approach: thinning Poisson processes
    • The number of islands
    • Proportion of coverage
    • # clones in an island
    • The length of an island
  • Actual vs apparent islands
    • Variant 1: Partial overlap detection
    • Variant 2: Anchoring random clones
    • Variant 3: Directed mapping
    • Variant 4: Double-end-sequencing
• STS-content mapping
  • Distance model: Maximum likelihood approach
• References
• About this document ...