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Biological details of the gene expression mechanism

How is information encoded in DNA used? Central to understanding this is figuring out gene expression mechanisms. The main idea is that every gene contains the information to produce a protein, which is a linear arrangement of amino acids. There are 20 types of amino acids used in proteins, so our task is to figure out how a sequence of in DNA is converted to a sequence in the amino acid alphabet. The really interesting (and hard) part is learning what triggers and regulates gene expression.

The story is slightly different for prokaryotic and eukaryotic organisms . Prokaryotes lack a nucleus in their cells and their genome is floating around somewhere in the cell, while eukaryotes have a nucleus in which the genome is contained. The basic story of gene expression can be divided into two stages, transcription and translation. In the first stage, DNA is transcribed to produce RNA (ribonucleic acid), in the second stage the RNA is translated to produce a protein. As far as we are concerned, the difference between RNA and DNA is that RNA is usually a single stranded molecule, and the nucleotides it uses are where Thymine used in DNA is replaced by Uracil. This intermediary RNA is know as messenger RNA (mRNA for short).





Simon Cawley
Fri May 1 15:50:13 PDT 1998